Visualizing SNVs to quantify allele-specific expression in single cells.
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ABSTRACT:
SUBMITTER: Levesque MJ
PROVIDER: S-EPMC3771873 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Visualizing SNVs to quantify allele-specific expression in single cells.
Levesque Marshall J MJ Ginart Paul P Wei Yichen Y Raj Arjun A
Nature methods 20130804 9
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells. ...[more]
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