Ontology highlight
ABSTRACT:
SUBMITTER: Callegari E
PROVIDER: S-EPMC3771938 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Callegari Elisa E Elamin Bahaeldin K BK D'Abundo Lucilla L Falzoni Simonetta S Donvito Giovanna G Moshiri Farzaneh F Milazzo Maddalena M Altavilla Giuseppe G Giacomelli Luciano L Fornari Francesca F Hemminki Akseli A Di Virgilio Francesco F Gramantieri Laura L Negrini Massimo M Sabbioni Silvia S
PloS one 20130912 9
The down-regulation of miR-199 occurs in nearly all primary hepatocellular carcinomas (HCCs) and HCC cell lines in comparison with normal liver. We exploited this miR-199 differential expression to develop a conditionally replication-competent oncolytic adenovirus, Ad-199T, and achieve tumor-specific viral expression and replication. To this aim, we introduced four copies of miR-199 target sites within the 3' UTR of E1A gene, essential for viral replication. As consequence, E1A expression from A ...[more]