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Nanoparticle for delivery of antisense ?PNA oligomers targeting CCR5.


ABSTRACT: The development of a new class of peptide nucleic acids (PNAs), i.e., gamma PNAs (?PNAs), creates the need for a general and effective method for its delivery into cells for regulating gene expression in mammalian cells. Here we report the antisense activity of a recently developed hydrophilic and biocompatible diethylene glycol (miniPEG)-based gamma peptide nucleic acid called MP?PNAs via its delivery by poly(lactide-co-glycolide) (PLGA)-based nanoparticle system. We show that MP?PNA oligomers designed to bind to the selective region of chemokine receptor 5 (CC R5) transcript, induce potent and sequence-specific antisense effects as compared with regular PNA oligomers. In addition, PLGA nanoparticle delivery of MP?PNAs is not toxic to the cells. The findings reported in this study provide a combination of ?PNA technology and PLGA-based nanoparticle delivery method for regulating gene expression in live cells via the antisense mechanism.

SUBMITTER: Bahal R 

PROVIDER: S-EPMC3771998 | biostudies-literature | 2013 Apr-Jun

REPOSITORIES: biostudies-literature

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Nanoparticle for delivery of antisense γPNA oligomers targeting CCR5.

Bahal Raman R   McNeer Nicole Ali NA   Ly Danith H DH   Saltzman W Mark WM   Glazer Peter M PM  

Artificial DNA, PNA & XNA 20130401 2


The development of a new class of peptide nucleic acids (PNAs), i.e., gamma PNAs (γPNAs), creates the need for a general and effective method for its delivery into cells for regulating gene expression in mammalian cells. Here we report the antisense activity of a recently developed hydrophilic and biocompatible diethylene glycol (miniPEG)-based gamma peptide nucleic acid called MPγPNAs via its delivery by poly(lactide-co-glycolide) (PLGA)-based nanoparticle system. We show that MPγPNA oligomers  ...[more]

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