Fc? receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies.

ABSTRACT: Monocytes/macrophages are potent mediators of antitumor antibody therapy, where they engage target cells via Fc? receptors (Fc?R). Binding of these cells to opsonized tumor targets elicits cytokine production, phagocytosis, and antibody-mediated cellular cytotoxicity. Here we show for the first time that activation of monocyte Fc?R results in the secretion of soluble vascular endothelial growth factor receptor-1 (VEGFR-1/sFlt-1), which serves to antagonize VEGF-mediated angiogenesis and tumor growth. Consistent with this, using a murine solid tumor model of antibody therapy, we show that sFlt-1 is involved in restricting tumor growth. Analyzing the mechanism of induction of sFlt-1, we found that the Erk and PI3K pathways were required for transcription, and NF-?B was required for translation. Upon closer examination of the role of NF-?B, we found that a microRNA, miR181a, negatively regulates Fc?R-mediated sFlt-1 production and that NF-?B serves to antagonize this microRNA. Taken together, these results demonstrate a novel and biologically important function of monocytes and macrophages during antibody therapy.

SUBMITTER: Justiniano SE 

PROVIDER: S-EPMC3772225 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature