SeedSeq: off-target transcriptome database.
Ontology highlight
ABSTRACT:
SUBMITTER: Das S
PROVIDER: S-EPMC3773410 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
SeedSeq: off-target transcriptome database.
Das Shaoli S Ghosal Suman S Chakrabarti Jayprokas J Kozak Karol K
BioMed research international 20130829
Detection of potential cross-reaction between a short oligonucleotide sequence and a longer (unintended) sequence is crucial for many biological applications, such as high content screening (HCS), microarray nucleotide probes, or short interfering RNAs (siRNAs). However, owing to a tolerance for mismatches and gaps in base-pairing with target transcripts, siRNAs could have up to hundreds of potential target sequences in a genome, and some small RNAs in mammalian systems have been shown to affect ...[more]
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