Ontology highlight
ABSTRACT:
SUBMITTER: Mellacheruvu D
PROVIDER: S-EPMC3773500 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Mellacheruvu Dattatreya D Wright Zachary Z Couzens Amber L AL Lambert Jean-Philippe JP St-Denis Nicole A NA Li Tuo T Miteva Yana V YV Hauri Simon S Sardiu Mihaela E ME Low Teck Yew TY Halim Vincentius A VA Bagshaw Richard D RD Hubner Nina C NC Al-Hakim Abdallah A Bouchard Annie A Faubert Denis D Fermin Damian D Dunham Wade H WH Goudreault Marilyn M Lin Zhen-Yuan ZY Badillo Beatriz Gonzalez BG Pawson Tony T Durocher Daniel D Coulombe Benoit B Aebersold Ruedi R Superti-Furga Giulio G Colinge Jacques J Heck Albert J R AJ Choi Hyungwon H Gstaiger Matthias M Mohammed Shabaz S Cristea Ileana M IM Bennett Keiryn L KL Washburn Mike P MP Raught Brian B Ewing Rob M RM Ewing Rob M RM Gingras Anne-Claude AC Nesvizhskii Alexey I AI
Nature methods 20130707 8
Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona fide interactors versus those that are background contaminants (for example, proteins that interact with the solid-phase support, affinity reagent or epitope tag) is a challenging task. The standard approach is to identify nonspecific interactions u ...[more]