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Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system.

ABSTRACT: A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.

SUBMITTER: Tomura M

PROVIDER: S-EPMC3774768 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system.

Tomura Michio M Sakaue-Sawano Asako A Mori Yoshiko Y Takase-Utsugi Mitsuyo M Hata Akihiro A Ohtawa Kenji K Kanagawa Osami O Miyawaki Atsushi A

PloS one 20130916 9

A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and ...[more]

PMID: 24066072

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