Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag.

ABSTRACT: The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.

SUBMITTER: Gatzogiannis E 

PROVIDER: S-EPMC3775488 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature