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Arabidopsis C-terminal domain phosphatase-like 1 functions in miRNA accumulation and DNA methylation.

ABSTRACT: Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated for gene regulation mediated by dsRNA-dependent pathways. We investigated the role of CPL1 and its dsRBMs in various gene silencing pathways. Genetic interaction analyses revealed that cpl1 was able to partially suppress transcriptional gene silencing and DNA hypermethylation phenotype of ros1 suggesting CPL1 is involved in the RNA-directed DNA methylation pathway without reducing siRNA production. By contrast, cpl1 reduced some miRNA levels at the level of processing. Indeed, CPL1 protein interacted with proteins important for miRNA biogenesis, suggesting that CPL1 regulates miRNA processing. These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway.

SUBMITTER: Jeong IS 

PROVIDER: S-EPMC3776750 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Arabidopsis C-terminal domain phosphatase-like 1 functions in miRNA accumulation and DNA methylation.

Jeong In Sil IS   Aksoy Emre E   Fukudome Akihito A   Akhter Salina S   Hiraguri Akihiro A   Fukuhara Toshiyuki T   Bahk Jeong Dong JD   Koiwa Hisashi H  

PloS one 20130918 9

Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated for gene regulation mediated by dsRNA-dependent pathways. We investigated the role of CPL1 and its dsRBMs in various gene silencing pathways. Genetic interaction analyses revealed that cpl1 was able to partially suppress transcriptional gene sil  ...[more]

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