Proteome response to the panneural expression of human wild-type alpha-synuclein: a Drosophila model of Parkinson's disease.

ABSTRACT: The alpha-synuclein protein is associated with several neurodegenarative diseases, including Parkinson's disease (PD). In humans, only mutated forms of alpha-synuclein are linked to PD; however, panneural expression of human wild-type (WT) alpha-synuclein induces Parkinson's like-symptoms in Drosophila. Here, we report a quantitative proteomic analysis of WT alpha-synuclein transgenic flies with age-matched controls at the presymptomatic stage utilizing a global isotopic labeling strategy combined with multidimensional liquid chromatographies and tandem mass spectrometry. The analysis includes two biological replicates, in which samples are isotopically labeled in forward and reverse directions. In total, 229 proteins were quantified from assignments of at least two peptide sequences. Of t

SUBMITTER: Xun Z 

PROVIDER: S-EPMC3780383 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature