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Development of semi-defined rice straw-based medium for butanol production and its kinetic study.

ABSTRACT: Rice straw is one of the potential economic feedstock for biobutanol production through ABE fermentation. However, the rice straw hydrolysate-based fermentation medium needs to be supported with nutritional elements. In this study, an attempt is made to optimize the rice straw hydrolysate-based fermentation medium employing Clostridium acetobutylicum MTCC 481 using Taguchi design of experiments (DOE) statistical model. Initially, a set of 12 nutrient components viz. MgNO₃·6H₂O, FeNO₃, NH₄NO₃, yeast extract, PABA, biotin, PABA + biotin mixture, CaCl₂, KCl, NaCl, MgSO₄ and CH₃COONa were screened through classical (one-variable-at-a-time) method. Based on the results, four components (PABA, yeast extract, MgSO₄ and CH₃COONa) were found to have significant impact, and were further subjected to statistical optimization through Taguchi DOE method. These experiments revealed that RSH supported with 3 g L^-1 of yeast extract and 4 mg L^-1 PABA to RSH was the most optimum fermentation medium. Experiments using 2 L bioreactor with this optimum fermentation medium showed nearly complete utilization of soluble sugars with the production of 8.7 g L^-1 of total solvents and 6 g L^-1 of butanol. The experimental data were fitted to kinetic models reported in the literature to determine the kinetic parameters of the fermentation process. An interesting result was revealed from this analysis that the under optimized fermentation medium, the kinetic parameters for both shake flask and bioreactor level were similar. This essentially means that effect of scale of operation is rendered insignificant when fermentation medium is under optimum conditions.

SUBMITTER: Ranjan A

PROVIDER: S-EPMC3781261 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Development of semi-defined rice straw-based medium for butanol production and its kinetic study.

Ranjan Amrita A Mayank Rahul R Moholkar Vijayanand S VS

3 Biotech 20130309 5

Rice straw is one of the potential economic feedstock for biobutanol production through ABE fermentation. However, the rice straw hydrolysate-based fermentation medium needs to be supported with nutritional elements. In this study, an attempt is made to optimize the rice straw hydrolysate-based fermentation medium employing Clostridium acetobutylicum MTCC 481 using Taguchi design of experiments (DOE) statistical model. Initially, a set of 12 nutrient components viz. MgNO<sub>3</sub>·6H<sub>2</su ...[more]

PMID: 28324335

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Project description:BackgroundThe marine thermophilic bacterium Rhodothermus marinus can degrade many polysaccharides which makes it interesting as a future cell factory. Progress using this bacterium has, however, been hampered by limited knowledge on media and conditions for biomass production, often resulting in low cell yields and low productivity, highlighting the need to develop conditions that allow studies of the microbe on molecular level. This study presents development of defined conditions that support growth, combined with evaluation of production of carotenoids and exopolysaccharides (EPSs) by R. marinus strain DSM 16675.ResultsTwo defined media were initially prepared: one including a low addition of yeast extract (modified Wolfe's medium) and one based on specific components (defined medium base, DMB) to which two amino acids (N and Q), were added. Cultivation trials of R. marinus DSM 16675 in shake flasks, resulted in maximum cell densities (OD620 nm) of 2.36 ± 0.057, cell dry weight (CDW) 1.2 ± 0.14 mg/L, total carotenoids 0.59 × 10-3 mg/L, and EPSs 1.72 ± 0.03 mg/L using 2 g/L glucose in DMB. In Wolfe's medium (supplemented by 0.05 g/L yeast extract and 2.5 g/L glucose), maximum OD620 nm was 2.07 ± 0.05, CDW 1.05 ± 0.07 mg/L, total carotenoids 0.39 × 10-3 mg/L, and EPSs 1.74 ± 0.2 mg/L. Growth trials at 5 g/L glucose in these media either failed or resulted in incomplete substrate utilization. To improve reproducibility and increase substrate utilization, a screening of macroelements (e.g. phosphate) in DMB, was combined with use of trace elements and vitamins of the modified Wolfe's medium. The resulting defined minimal R. marinus medium, (DRM), allowed reproducible cultivations to a final OD620nm of 6.6 ± 0.05, CDW 2.85 ± 0.07 mg/L, a maximum specific growth rate (µmax) of 0.26 h-1, total carotenoids 0.77 × 10-3 mg/L and EPSs 3.4 ± 0.17 mg/L in cultivations supplemented with up to 5 g/L glucose.ConclusionA minimal defined medium (DRM) was designed that resulted in reproducible growth and an almost doubled formation of both total carotenoids and EPSs. Such defined conditions, are necessary for systematic studies of metabolic pathways, to determine the specific requirements for growth and fully characterize metabolite production.

Dataset Information

Development of semi-defined rice straw-based medium for butanol production and its kinetic study.

Publications

Development of semi-defined rice straw-based medium for butanol production and its kinetic study.

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