Insight into the family of Na+/Ca2+ exchangers of Caenorhabditis elegans.
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ABSTRACT:
SUBMITTER: Sharma V
PROVIDER: S-EPMC3781985 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:We report that CGP37157 improved the evolution with age of the sarcomeric regular structure, delaying development of sarcopenia in C. elegans body wall muscle. Similarly, CGP37157 favoured the maintenance of a regular mitochondrial structure during aging. CGP37157 induced a four-fold increase in the expression of ncx-6, one of the C. elegans mitochondrial Na+/Ca2+ exchangers.
2021-05-01 | GSE173646 | GEO
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Project description:In the nematode Caenorhabditis elegans, fem-1, fem-2, and fem-3 play crucial roles in male sexual development. Among these three genes, fem-2 encodes a PP2C (serine/threonine phosphatase type 2C)-like protein, whose activity promotes the development of masculinity. Different from the canonical PP2Cs, FEM-2 consists of an additional N-terminal domain (NTD) apart from its C-terminal catalytic domain. Interestingly, genetic studies have indicated indispensable roles for both of these two domains of FEM-2 in promoting male development, but the underlying mechanism remains unknown. In the present study, we solved the crystal structure of full-length FEM-2, which revealed a novel structural fold formed by its NTD. Structural and functional analyses demonstrated that the NTD did not directly regulate the in vitro dephosphorylation activity of FEM-2, but instead functioned as a scaffold domain in the assembly of the FEM-1/2/3 complex, the executioner in the final step of the sex determination pathway. Biochemical studies further identified the regions in the NTD involved in FEM-1 and FEM-3 interactions. Our results not only identified a novel fold formed by the extra domain of a noncanonical PP2C enzyme, but also provided important insights into the molecular mechanism of how the NTD works in mediating the sex-determining role of FEM-1/2/3 complex.
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