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Ultrastructural abnormalities in CA1 hippocampus caused by deletion of the actin regulator WAVE-1.

ABSTRACT: By conveying signals from the small GTPase family of proteins to the Arp2/3 complex, proteins of the WAVE family facilitate actin remodeling. The WAVE-1 isoform is expressed at high levels in brain, where it plays a role in normal synaptic processing, and is implicated in hippocampus-dependent memory retention. We used electron microscopy to determine whether synaptic structure is modified in the hippocampus of WAVE-1 knockout mice, focusing on the neuropil of CA1 stratum radiatum. Mice lacking WAVE-1 exhibited alterations in the morphology of both axon terminals and dendritic spines; the relationship between the synaptic partners was also modified. The abnormal synaptic morphology we observed suggests that signaling through WAVE-1 plays a critical role in establishing normal synaptic architecture in the rodent hippocampus.

SUBMITTER: Hazai D

PROVIDER: S-EPMC3783472 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Ultrastructural abnormalities in CA1 hippocampus caused by deletion of the actin regulator WAVE-1.

Hazai Diána D Szudoczki Róbert R Ding Jindong J Soderling Scott H SH Weinberg Richard J RJ Sótonyi Péter P Rácz Bence B

PloS one 20130925 9

By conveying signals from the small GTPase family of proteins to the Arp2/3 complex, proteins of the WAVE family facilitate actin remodeling. The WAVE-1 isoform is expressed at high levels in brain, where it plays a role in normal synaptic processing, and is implicated in hippocampus-dependent memory retention. We used electron microscopy to determine whether synaptic structure is modified in the hippocampus of WAVE-1 knockout mice, focusing on the neuropil of CA1 stratum radiatum. Mice lacking ...[more]

PMID: 24086480

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