Ontology highlight
ABSTRACT:
SUBMITTER: Goldson-Barnaby A
PROVIDER: S-EPMC3789393 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Enzyme research 20130912
Trichosporon cutaneum phenylalanine ammonia lyase was selected as a model to investigate the dual substrate activity of this family of enzymes. Sequencing of the PAL gene identified an extensive intron region at the N-terminus. Five amino acid residues differing from a prior report were identified. Highest Phe : Tyr activities (1.6 ± 0.3 : 0.4 ± 0.1 μ mol/h g wet weight) were induced by Tyr. The enzyme has a temperature optimum of 32°C and a pH optimum of 8-8.5 and shows no metal cofactor depe ...[more]