Dataset Information

The contribution of non-conventional T cells and NK cells in the mycobacterial-specific IFN? response in Bacille Calmette-Guerin (BCG)-immunized infants.

ABSTRACT:

Background

The Mycobacterium bovis Bacille Calmette-Guérin (BCG) vaccine is given to >120 million infants each year worldwide. Most studies investigating the immune response to BCG have focused on adaptive immunity. However the importance of TCR-gamma/delta (??) T cells and NK cells in the mycobacterial-specific immune response is of increasing interest.

Methods

Participants in four age-groups were BCG-immunized. Ten weeks later, in vitro BCG-stimulated blood was analyzed for NK and T cell markers, and intracellular IFNgamma (IFN?) by flow cytometry. Total functional IFN? response was calculated using integrated median fluorescence intensity (iMFI).

Results

In infants and children, CD4 and CD4-CD8- (double-negative (DN)) T cells were the main IFN?-expressing cells representing 43-56% and 27-37% of total CD3+ IFN?+ T cells respectively. The iMFI was higher in DN T cells compared to CD4 T cells in all age groups, with the greatest differences seen in infants immunized at birth (p=0.002) or 2 months of age (p<0.0001). When NK cells were included in the analysis, they accounted for the majority of total IFN?-expressing cells and, together with DN V?2 ?? T cells, had the highest iMFI in infants immunized at birth or 2 months of age.

Conclusion

In addition to CD4 T cells, NK cells and DN T cells, including V?2 ?? T cells, are the key populations producing IFN? in response to BCG immunization in infants and children. This suggests that innate immunity and unconventional T cells play a greater role in the mycobacterial immune response than previously recognized and should be considered in the design and assessment of novel tuberculosis vaccines.

SUBMITTER: Zufferey C

PROVIDER: S-EPMC3789697 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

The contribution of non-conventional T cells and NK cells in the mycobacterial-specific IFNγ response in Bacille Calmette-Guérin (BCG)-immunized infants.

Zufferey Christel C Germano Susie S Dutta Binita B Ritz Nicole N Curtis Nigel N

PloS one 20131003 10

<h4>Background</h4>The Mycobacterium bovis Bacille Calmette-Guérin (BCG) vaccine is given to >120 million infants each year worldwide. Most studies investigating the immune response to BCG have focused on adaptive immunity. However the importance of TCR-gamma/delta (γδ) T cells and NK cells in the mycobacterial-specific immune response is of increasing interest.<h4>Methods</h4>Participants in four age-groups were BCG-immunized. Ten weeks later, in vitro BCG-stimulated blood was analyzed for NK a ...[more]

PMID: 24098583

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