Ontology highlight
ABSTRACT:
SUBMITTER: Lukina MV
PROVIDER: S-EPMC3789988 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Lukina Maria V MV Popov Alexander V AV Koval Vladimir V VV Vorobjev Yuri N YN Fedorova Olga S OS Zharkov Dmitry O DO
The Journal of biological chemistry 20130817 40
8-Oxoguanine-DNA glycosylase (OGG1) removes premutagenic lesion 8-oxoguanine (8-oxo-G) from DNA and then nicks the nascent abasic (apurinic/apyrimidinic) site by β-elimination. Although the structure of OGG1 bound to damaged DNA is known, the dynamic aspects of 8-oxo-G recognition are not well understood. To comprehend the mechanisms of substrate recognition and processing, we have constructed OGG1 mutants with the active site occluded by replacement of Cys-253, which forms a wall of the base-bi ...[more]