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Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models.


ABSTRACT: The utilization of 3D, physiologically relevant in vitro cancer models to investigate complex interactions between tumor and stroma has been increasing. Prior work has generally focused on the cancer cells and, the role of fibroblast culture conditions on tumor-stromal cell interactions is still largely unknown. Here, we focus on the stroma by comparing functional behaviors of human mammary fibroblasts (HMFs) cultured in 2D and 3D and their effects on the invasive progression of breast cancer cells (MCF10DCIS.com). We identified increased levels of several paracrine factors from HMFs cultured in 3D conditions that drive the invasive transition. Using a microscale co-culture model with improved compartmentalization and sensitivity, we demonstrated that HMFs cultured in 3D intensify the promotion of the invasive progression through the HGF/c-Met interaction. This study highlights the importance of the 3D stromal microenvironment in the development of multiple cell type in vitro cancer models.

SUBMITTER: Sung KE 

PROVIDER: S-EPMC3790689 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models.

Sung Kyung Eun KE   Su Xiaojing X   Berthier Erwin E   Pehlke Carolyn C   Friedl Andreas A   Beebe David J DJ  

PloS one 20131004 10


The utilization of 3D, physiologically relevant in vitro cancer models to investigate complex interactions between tumor and stroma has been increasing. Prior work has generally focused on the cancer cells and, the role of fibroblast culture conditions on tumor-stromal cell interactions is still largely unknown. Here, we focus on the stroma by comparing functional behaviors of human mammary fibroblasts (HMFs) cultured in 2D and 3D and their effects on the invasive progression of breast cancer ce  ...[more]

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