Ontology highlight
ABSTRACT:
SUBMITTER: Klappe K
PROVIDER: S-EPMC3791605 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Klappe Karin K Dijkhuis Anne-Jan AJ Hummel Ina I van Dam Annie A Ivanova Pavlina T PT Milne Stephen B SB Myers David S DS Brown H Alex HA Permentier Hjalmar H Kok Jan W JW
The Biochemical journal 20100901 3
We show that highly efficient depletion of sphingolipids in two different cell lines does not abrogate the ability to isolate Lubrol-based DRMs (detergent-resistant membranes) or detergent-free lipid rafts from these cells. Compared with control, DRM/detergent-free lipid raft fractions contain equal amounts of protein, cholesterol and phospholipid, whereas the classical DRM/lipid raft markers Src, caveolin-1 and flotillin display the same gradient distribution. DRMs/detergent-free lipid rafts th ...[more]