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Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster.


ABSTRACT: Succinyl-CoA:3-ketoacid CoA transferase (SCOT) plays a crucial role in ketone-body metabolism. SCOT from Drosophila melanogaster (DmSCOT) was purified and crystallized. The crystal structure of DmSCOT was determined at 2.64?Å resolution and belonged to space group P212121, with unit-cell parameters a=76.638, b=101.921, c=122.457?Å, ?=?=?=90°. Sequence alignment and structural analysis identified DmSCOT as a class I CoA transferase. Compared with Acetobacter aceti succinyl-CoA:acetate CoA transferase, DmSCOT has a different substrate-binding pocket, which may explain the difference in their substrate specificities.

SUBMITTER: Zhang M 

PROVIDER: S-EPMC3792662 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster.

Zhang Min M   Xu Han-Yang HY   Wang Yi-Cui YC   Shi Zhu-Bing ZB   Zhang Nan-Nan NN  

Acta crystallographica. Section F, Structural biology and crystallization communications 20130928 Pt 10


Succinyl-CoA:3-ketoacid CoA transferase (SCOT) plays a crucial role in ketone-body metabolism. SCOT from Drosophila melanogaster (DmSCOT) was purified and crystallized. The crystal structure of DmSCOT was determined at 2.64 Å resolution and belonged to space group P212121, with unit-cell parameters a=76.638, b=101.921, c=122.457 Å, α=β=γ=90°. Sequence alignment and structural analysis identified DmSCOT as a class I CoA transferase. Compared with Acetobacter aceti succinyl-CoA:acetate CoA transfe  ...[more]

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