Ontology highlight
ABSTRACT:
SUBMITTER: Miao X
PROVIDER: S-EPMC3792675 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Miao Xiangzhi X Huang Xianhui X Zhang Guofang G Zhao Xiufang X Zhu Xianming X Dong Hui H
Acta crystallographica. Section F, Structural biology and crystallization communications 20130928 Pt 10
(2R,3R)-2,3-Butanediol dehydrogenase (R,R-BDH) from Bacillus coagulans 2-6 is a zinc-dependent medium-chain alcohol dehydrogenase. Recombinant R,R-BDH with a His6 tag at the C-terminus was expressed in Escherichia coli BL21 (DE3) cells and purified by Ni2+-chelating affinity and size-exclusion chromatography. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K. The crystallization condition consisted of 8%(v/v) Tacsimate pH 4.6, 18%(w/v) polyethylene glycol 3350. The crystal ...[more]