Dataset Information

Taxonomic and molecular identification of hemicaloosia, hemicycliophora, gracilacus and paratylenchus species (nematoda: criconematidae).

ABSTRACT: Populations of Hemicycliophora epicharoides, H. gigas, H. labiata, H. pruni, H. shepherdi, H. vidua, H. zuckermani, Gracilacus straeleni, and Paratylenchus labiosus were obtained from different geographical areas in the continental United States and characterized morphological and molecularly. Two new species of Hemicycliophorinae: Hemicaloosia uarki n. sp from Pinetree, St. Francis County, Arkansas, and Hemicycliophora wyei n. sp from Wayne County, North Carolina, are also described. Hemicaloosia uarki n. sp. is characterized by having two lip annuli separated from the rest of body and directed anteriorly, a long stylet (106-124 ?m), long body length (1,081-1,326 ?m) and a single lateral fields demarcated by interruptions of the body annuli. Hemicycliophora wyei n. sp. showed a lateral fields demarked by two faint lines with transverse anastomoses and/or breaks of the striae; an elongated not offset conical tail with distinct annulations and a rounded tip and long vulval lips with a vulval sleeve. The molecular characterizations of the new (H. uarki n. sp. and H. wyei n. sp.) and known species of Criconematidae using the ITS1 rDNA gene sequence and the molecular phylogenetic relationships are provided.

SUBMITTER: Cordero Lopez MA

PROVIDER: S-EPMC3792835 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Taxonomic and molecular identification of hemicaloosia, hemicycliophora, gracilacus and paratylenchus species (nematoda: criconematidae).

Cordero López Marco A MA Robbins Robert T RT Szalanski Allen L AL

Journal of nematology 20130901 3

Populations of Hemicycliophora epicharoides, H. gigas, H. labiata, H. pruni, H. shepherdi, H. vidua, H. zuckermani, Gracilacus straeleni, and Paratylenchus labiosus were obtained from different geographical areas in the continental United States and characterized morphological and molecularly. Two new species of Hemicycliophorinae: Hemicaloosia uarki n. sp from Pinetree, St. Francis County, Arkansas, and Hemicycliophora wyei n. sp from Wayne County, North Carolina, are also described. Hemicaloos ...[more]

PMID: 24115782

Similar Datasets

Project description:The family Criconematidae is a remarkable group of nematodes, containing roughly 600 nominal root-ectoparasitic species, of which many species are known to be significant agricultural pests. Strikingly, our phylogenetic analyses based on 18S, D2-D3 of 28S rRNA, and COI mtDNA sequences of criconematid species, supported by tree topology tests (SH and AU tests), revealed that almost all studied genera, including Criconema, Ogma, Crossonema, Discocriconema, Hemicriconemoides, Criconemoides, Mesocriconema, and Lobocriconema, are not monophyletic groups, a finding that is partly contrary to those of previous studies on these groups. Our results suggest that key morphological characters used in the classification of Criconematidae are the consequence of convergent evolution. It is clear from our studies that the species status of at least 40 sequences of criconematid species from GenBank must be either revised or reconsidered, with analyses based on a polyphasic approach that includes different tree- and distance-based molecular species-delimitation methods (bPTP, GMYC, ABGD1, and ABGD2). Our studies found the ABGD2 output of the automatic barcode method to agree remarkably well with established species delimitations, while in general, the four species-delimitation results corresponding to three barcode regions forwarded significantly more putative species compared to those originally considered. This study also characterised for the first time the populations of Criconemoides myungsugae and Discocriconemella hensungica associated with Vietnamese ginseng, one of the most precious and rare ginseng varieties in the world. Although these populations are morphologically in agreement with the original descriptions of C. myungsugae and D. hengsungica, their molecular data display notable variations compared to the sequences deposited in GenBank. These species demonstrate clearly the immense molecular variations that can be observed in several species of the family Criconematidae.

Project description:Mesocriconema abolafiai n. sp. is described by morphological, morphometric, and molecular approaches. The new species is characterized by a body slightly curved with 402 to 612 μm length, 90 to 113 cuticular body annuli with smooth to irregular margins lacking of crenation with not more than one anastomoses, lip region not offset, small flattened submedian lobes, stylet robust (52.8-60.0 µm) with well-developed knobs, open vulva with simple anterior lip, straight vagina, filled spermatheca with globular sperms, presence of males, and conical-acute tail with last annulus bilobed or rounded. Discussions are made on the characterization of M. abolafiai n. sp. from the most closely related species, M. ozarkiense, and several other species having similar tail shape. Furthermore, results of phylogenetic analyses inferred from D2 to D3 expansion fragments of 28S rRNA, 18S rRNA, and ITS rRNA gene sequences revealed the phylogenetic position of the new species within representatives of Criconematidae and supported morphological justifications for considering this population from Iran as a new species in the genus Mesocriconema.Mesocriconema abolafiai n. sp. is described by morphological, morphometric, and molecular approaches. The new species is characterized by a body slightly curved with 402 to 612 μm length, 90 to 113 cuticular body annuli with smooth to irregular margins lacking of crenation with not more than one anastomoses, lip region not offset, small flattened submedian lobes, stylet robust (52.8-60.0 µm) with well-developed knobs, open vulva with simple anterior lip, straight vagina, filled spermatheca with globular sperms, presence of males, and conical-acute tail with last annulus bilobed or rounded. Discussions are made on the characterization of M. abolafiai n. sp. from the most closely related species, M. ozarkiense, and several other species having similar tail shape. Furthermore, results of phylogenetic analyses inferred from D2 to D3 expansion fragments of 28S rRNA, 18S rRNA, and ITS rRNA gene sequences revealed the phylogenetic position of the new species within representatives of Criconematidae and supported morphological justifications for considering this population from Iran as a new species in the genus Mesocriconema.

Project description:In Winter 2016, root and soil samples were collected from the rhizosphere soil at 10 to 15?cm depth of turfgrass, in the yard of a complex of houses in Caxias, a region near Lisbon, Portugal. The grass (dominated by tall fescue) showed yellow patches, stunting, and poor growth. Several specimens of a ring nematode (50-60 nematodes/100?ml soil) were recovered from soil and identified as Mesocriconema xenoplax (peach ring nematode) based on morphological and morphometrical analysis of females. The observed morphological features were with previous descriptions. Species identification was confirmed through sequencing of the fragment spanning D2/D3 domain of the 28S rDNA gene. Phylogenetic analysis revealed that the Portuguese isolate grouped with M. xenoplax isolates (98% similarity), supporting its identification as M. xenoplax. This is the first report of M. xenoplax from turfgrass in Portugal and in Europe contributing with additional information on the distribution of this phytoparasite.In Winter 2016, root and soil samples were collected from the rhizosphere soil at 10 to 15?cm depth of turfgrass, in the yard of a complex of houses in Caxias, a region near Lisbon, Portugal. The grass (dominated by tall fescue) showed yellow patches, stunting, and poor growth. Several specimens of a ring nematode (50-60 nematodes/100?ml soil) were recovered from soil and identified as Mesocriconema xenoplax (peach ring nematode) based on morphological and morphometrical analysis of females. The observed morphological features were with previous descriptions. Species identification was confirmed through sequencing of the fragment spanning D2/D3 domain of the 28S rDNA gene. Phylogenetic analysis revealed that the Portuguese isolate grouped with M. xenoplax isolates (98% similarity), supporting its identification as M. xenoplax. This is the first report of M. xenoplax from turfgrass in Portugal and in Europe contributing with additional information on the distribution of this phytoparasite.

Dataset Information

Taxonomic and molecular identification of hemicaloosia, hemicycliophora, gracilacus and paratylenchus species (nematoda: criconematidae).

Publications

Taxonomic and molecular identification of hemicaloosia, hemicycliophora, gracilacus and paratylenchus species (nematoda: criconematidae).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets