Unknown

Dataset Information

0

The effects of hematopoietic growth factors on neurite outgrowth.


ABSTRACT: Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are initially discovered as the essential hematopoietic growth factors regulating bone marrow stem cell proliferation and differentiation, and SCF in combination with G-CSF (SCF+G-CSF) has synergistic effects on bone marrow stem cell mobilization. In this study we have determined the effect of SCF and G-CSF on neurite outgrowth in rat cortical neurons. Using molecular and cellular biology and live cell imaging approaches, we have revealed that receptors for SCF and G-CSF are expressed on the growth core of cortical neurons, and that SCF+G-CSF synergistically enhances neurite extension through PI3K/AKT and NFκB signaling pathways. Moreover, SCF+G-CSF induces much greater NFκB activation, NFκB transcriptional binding and brain-derived neurotrophic factor (BDNF) production than SCF or G-CSF alone. In addition, we have also observed that BDNF, the target gene of NFκB, is required for SCF+G-CSF-induced neurite outgrowth. These data suggest that SCF+G-CSF has synergistic effects to promote neurite growth. This study provides new insights into the contribution of hematopoietic growth factors in neuronal plasticity.

SUBMITTER: Su Y 

PROVIDER: S-EPMC3792965 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

altmetric image

Publications

The effects of hematopoietic growth factors on neurite outgrowth.

Su Ye Y   Cui Lili L   Piao Chunshu C   Li Bin B   Zhao Li-Ru LR  

PloS one 20131008 10


Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are initially discovered as the essential hematopoietic growth factors regulating bone marrow stem cell proliferation and differentiation, and SCF in combination with G-CSF (SCF+G-CSF) has synergistic effects on bone marrow stem cell mobilization. In this study we have determined the effect of SCF and G-CSF on neurite outgrowth in rat cortical neurons. Using molecular and cellular biology and live cell imaging approaches, w  ...[more]

Similar Datasets

| S-EPMC10656890 | biostudies-literature
| S-EPMC1838971 | biostudies-literature
| S-EPMC6546251 | biostudies-literature
| S-EPMC2841637 | biostudies-literature
| S-EPMC2748697 | biostudies-literature
| S-EPMC5327485 | biostudies-literature
| S-EPMC3016852 | biostudies-literature
| S-EPMC6740475 | biostudies-literature
| S-EPMC8695970 | biostudies-literature
| S-EPMC4420918 | biostudies-literature