Ontology highlight
ABSTRACT:
SUBMITTER: Geiling B
PROVIDER: S-EPMC3795761 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Geiling Benjamin B Vandal Guillaume G Posner Ada R AR de Bruyns Angeline A Dutchak Kendall L KL Garnett Samantha S Dankort David D
PloS one 20131011 10
The ability to express exogenous cDNAs while suppressing endogenous genes via RNAi represents an extremely powerful research tool with the most efficient non-transient approach being accomplished through stable viral vector integration. Unfortunately, since traditional restriction enzyme based methods for constructing such vectors are sequence dependent, their construction is often difficult and not amenable to mass production. Here we describe a non-sequence dependent Gateway recombination clon ...[more]