Direct confocal acquisition of fluorescence from X-gal staining on thick tissue sections.
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ABSTRACT:
SUBMITTER: Levitsky KL
PROVIDER: S-EPMC3796308 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
Direct confocal acquisition of fluorescence from X-gal staining on thick tissue sections.
Levitsky Konstantin L KL Toledo-Aral Juan José JJ López-Barneo José J Villadiego Javier J
Scientific reports 20131014
X-gal staining is a common procedure used in the histochemical monitoring of gene expression by light microscopy. However, this procedure does not permit the direct confocal acquisition of images, thus preventing the identification of labelled cells on the depth (Z) axis of tissue sections and leading sometimes to erroneous conclusions in co-localization and gene expression studies. Here we report a technique, based on X-gal fluorescence emission and mathematically-based optical correction, to o ...[more]
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