Isolation of RNA from equine peripheral blood cells: comparison of methods.
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ABSTRACT:
SUBMITTER: Jiang Z
PROVIDER: S-EPMC3797321 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Isolation of RNA from equine peripheral blood cells: comparison of methods.
Jiang Zibin Z Uboh Cornelius E CE Chen Jinwen J Soma Lawrence R LR
SpringerPlus 20130922
Gene expression studies in equine research involve the use of whole blood samples as a vital source of RNA. To determine the optimal method for RNA isolation from equine whole blood, we compared three RNA isolation strategies using different commercially available kits to evaluate the yield and quality of equine RNA. All 3 methods produced RNA with high quality. Though it did not produce the highest yield, combining the quality, yield and the need for the downstream application in our project, L ...[more]
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