Unknown

Dataset Information

0

Cobalt(III) as a stable and inert mediator ion between NTA and His6-tagged proteins.


ABSTRACT: Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.

SUBMITTER: Wegner SV 

PROVIDER: S-EPMC3798096 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2529454 | biostudies-literature
| S-EPMC2533260 | biostudies-literature
| S-EPMC5066248 | biostudies-literature
| S-EPMC3617960 | biostudies-literature
| S-EPMC5310270 | biostudies-literature
| S-EPMC5510008 | biostudies-other
| S-EPMC3089116 | biostudies-literature
| S-EPMC3120378 | biostudies-literature
| S-EPMC6020529 | biostudies-literature
| S-EPMC555969 | biostudies-literature