Ontology highlight
ABSTRACT:
SUBMITTER: Sharma S
PROVIDER: S-EPMC3799443 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Sharma Sunny S Yang Jun J Watzinger Peter P Kötter Peter P Entian Karl-Dieter KD
Nucleic acids research 20130802 19
Yeast 25S rRNA was reported to contain a single cytosine methylation (m(5)C). In the present study using a combination of RP-HPLC, mung bean nuclease assay and rRNA mutagenesis, we discovered that instead of one, yeast contains two m(5)C residues at position 2278 and 2870. Furthermore, we identified and characterized two putative methyltransferases, Rcm1 and Nop2 to be responsible for these two cytosine methylations, respectively. Both proteins are highly conserved, which correlates with the pre ...[more]