Dataset Information

Association Analysis of IL10, TNF-?, and IL23R-IL12RB2 SNPs with Behcet's Disease Risk in Western Algeria.

ABSTRACT: OBJECTIVE:We have conducted the first study of the association of interleukin (IL)-10, tumor necrosis factor alpha (TNF-?), and IL23R-IL12RB2 region single nucleotide polymorphisms (SNPs) with Behçet's disease (BD) in Western Algeria. METHODS:A total of 51 BD patients and 96 unrelated controls from West region of Algeria were genotyped by direct sequencing for 11 SNPs including 2 SNPs from the IL10 promoter [c.-819T?>?C (rs1800871), c.-592A?>?C (rs1800872)], 6 SNPs from the TNF-? promoter [c.-1211T?>?C (rs1799964), c.-1043C?>?A (rs1800630), c.-1037C?>?T (rs1799724), c.-556G?>?A (rs1800750), c.-488G?>?A (rs1800629), and c.-418G?>?A (rs361525)], and 3 SNPs from the IL23R-IL12RB2 region [g.67747415A?>?C (rs12119179), g.67740092G?>?A (rs11209032), and g.67760140T?>?C (rs924080)]. RESULTS:The minor alleles c.-819T and c.-592A were significantly associated with BD [odds ratio (OR)?=?2.18; 95% confidence interval (CI) 1.28-3.73, p?=?0.003]; whereas, there was weaker association between TNF-? promoter SNPs or IL23R-IL12RB2 region and disease risk. CONCLUSION:Unlike the TNF-? and the IL23R-IL12RB2 region SNPs, the two IL10 SNPs were strongly associated with BD. The -819T, and -592A alleles and the -819TT, -819CT, and -592AA and -592CA genotypes seem to be highly involved in the risk of developing of BD in the population of Western Algeria.

SUBMITTER: Khaib Dit Naib O

PROVIDER: S-EPMC3801160 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

Association Analysis of IL10, TNF-α, and IL23R-IL12RB2 SNPs with Behçet's Disease Risk in Western Algeria.

Khaib Dit Naib Ouahiba O Aribi Mourad M Idder Aicha A Chiali Amel A Sairi Hakim H Touitou Isabelle I Lefranc Gérard G Lefranc Gérard G Barat-Houari Mouna M

Frontiers in immunology 20131021

<h4>Objective</h4>We have conducted the first study of the association of interleukin (IL)-10, tumor necrosis factor alpha (TNF-α), and IL23R-IL12RB2 region single nucleotide polymorphisms (SNPs) with Behçet's disease (BD) in Western Algeria.<h4>Methods</h4>A total of 51 BD patients and 96 unrelated controls from West region of Algeria were genotyped by direct sequencing for 11 SNPs including 2 SNPs from the IL10 promoter [c.-819T > C (rs1800871), c.-592A > C (rs1800872)], 6 SNPs from the TNF-α ...[more]

PMID: 24151497

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Project description:Behçet's disease (BD) susceptibility had been associated with single-nucleotide polymorphisms (SNPs) in IL23R-IL12RB2, IL10, STAT4, or ERAP1 locus in Japanese, Turkish, Chinese, and other populations, but not in a Korean genome-wide association study (GWAS). We aimed to fine-map BD risk association of these four loci using extensive imputation and additional genotyping for replication.In the discovery phase, 369 patients with BD enrolled in the previous Korean GWAS and 2000 controls retrieved from a population-based cohort of healthy Koreans were imputed for their genotypes of all SNPs in the four loci using the Asian data of the 1000 Genomes Project as reference. For genotype imputation of ERAP1 SNPs, the adjacent ERAP2 SNPs were also covered. For the 10 most significantly associated SNPs (8 imputed and 2 GWAS-genotyped), an additional 84 patients with BD and 283 healthy controls were genotyped for replication. The results from the discovery and replication phases were pooled for meta-analysis using the Mantel-Haenszel test to estimate the odds ratio (OR) and 95% confidence interval (CI).An IL23R-IL12RB2 intergenic SNP rs1495965 was significantly associated with BD risk (OR (95% CI)?=?1.5 (1.3, 1.7), P?=?2.5?×?10-7) in the pooled meta-analysis of the discovery (1.4 (1.2, 1.7), P?=?4.9?×?10-7) and replication (1.9 (1.3, 2.6), P?=?6.0?×?10-4) phases. BD risk association was fine-mapped on the intergenic region rather than the two flanking genes, as rs1495966 and rs4655535, almost perfectly correlated with rs1495965 (r 2?=?0.99), were also located in the same intergenic region. Consistent with previous reports, the P values tended to be lower within IL23R than IL12RB2. On the other hand, several IL10 SNPs were suggested for association in the discovery phase but all failed in the replication phase. No SNP in ERAP1-ERAP2 and STAT4 was suggested even in the discovery phase.BD susceptibility association was fine-mapped on the intergenic region between IL23R and IL12RB2 as marked by three correlated SNPs, rs1495965, rs1495966, and rs4655535.

Dataset Information

Association Analysis of IL10, TNF-?, and IL23R-IL12RB2 SNPs with Behcet's Disease Risk in Western Algeria.

Publications

Association Analysis of IL10, TNF-α, and IL23R-IL12RB2 SNPs with Behçet's Disease Risk in Western Algeria.

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