Atomic force microscopy captures folded ribosome bound nascent chains.
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ABSTRACT:
SUBMITTER: Loksztejn A
PROVIDER: S-EPMC3804001 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
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Atomic force microscopy captures folded ribosome bound nascent chains.
Loksztejn Anna A Scholl Zackary Z Marszalek Piotr E PE
Chemical communications (Cambridge, England) 20121011 96
Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic "horse-shoe" shape of ankyrin-R emerging from the ribosome was captured. ...[more]
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