Technological advances in site-directed spin labeling of proteins.
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ABSTRACT:
SUBMITTER: Hubbell WL
PROVIDER: S-EPMC3805720 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
Technological advances in site-directed spin labeling of proteins.
Hubbell Wayne L WL López Carlos J CJ Altenbach Christian C Yang Zhongyu Z
Current opinion in structural biology 20130711 5
Molecular flexibility over a wide time range is of central importance to the function of many proteins, both soluble and membrane. Revealing the modes of flexibility, their amplitudes, and time scales under physiological conditions is the challenge for spectroscopic methods, one of which is site-directed spin labeling EPR (SDSL-EPR). Here we provide an overview of some recent technological advances in SDSL-EPR related to investigation of structure, structural heterogeneity, and dynamics of prote ...[more]
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