Unknown

Dataset Information

0

Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection.


ABSTRACT: The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.

SUBMITTER: Sourisseau M 

PROVIDER: S-EPMC3805793 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection.

Sourisseau Marion M   Goldman Orit O   He Wenqian W   Gori Jennifer L JL   Kiem Hans-Peter HP   Gouon-Evans Valerie V   Evans Matthew J MJ  

Gastroenterology 20130725 5


The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using  ...[more]

Similar Datasets

| S-EPMC3353869 | biostudies-other
| S-EPMC3255820 | biostudies-other
| S-EPMC4600668 | biostudies-literature
| S-EPMC3289320 | biostudies-literature
| S-SCDT-10_1038-S44319-024-00236-0 | biostudies-other
| S-EPMC2840462 | biostudies-literature
| S-EPMC7366275 | biostudies-literature
| S-EPMC5832748 | biostudies-literature
| S-EPMC7072243 | biostudies-literature
| S-EPMC5379564 | biostudies-literature