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Improvement of L-arabinose fermentation by modifying the metabolic pathway and transport in Saccharomyces cerevisiae.

ABSTRACT: The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075?h(-1), a maximum specific L-arabinose consumption rate of 0.61?g?h(-1)?g(-1) dry cell weight, and a promising ethanol yield of 0.43?g?g(-1) from L-arabinose fermentation.

SUBMITTER: Wang C

PROVIDER: S-EPMC3806156 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Improvement of L-arabinose fermentation by modifying the metabolic pathway and transport in Saccharomyces cerevisiae.

Wang Chengqiang C Shen Yu Y Zhang Yanyan Y Suo Fan F Hou Jin J Bao Xiaoming X

BioMed research international 20130930

The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h(-1), a maximum specific L-arabinose consumption rate of 0.61 g h(-1) g(-1) dry cell weight, and a ...[more]

PMID: 24195072

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Identification of Important Amino Acids in Gal2p for Improving the L-arabinose Transport and Metabolism in <i>Saccharomyces cerevisiae</i>.

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