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SOFI-based 3D superresolution sectioning with a widefield microscope.

ABSTRACT: BACKGROUND:Fluorescence-based biological imaging has been revolutionized by the recent introduction of superresolution microscopy methods. 3D superresolution microscopy, however, remains a challenge as its implementation by existing superresolution methods is non-trivial. METHODS:Here we demonstrate a facile and straightforward 3D superresolution imaging and sectioning of the cytoskeletal network of a fixed cell using superresolution optical fluctuation imaging (SOFI) performed on a conventional lamp-based widefield microscope. RESULTS AND CONCLUSION:SOFI's inherent sectioning capability effectively transforms a conventional widefield microscope into a superresolution 'confocal widefield' microscope.

SUBMITTER: Dertinger T

PROVIDER: S-EPMC3807127 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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SOFI-based 3D superresolution sectioning with a widefield microscope.

Dertinger Thomas T Xu Jianmin J Naini Omeed Foroutan OF Vogel Robert R Weiss Shimon S

Optical nanoscopy 20121201 2

<h4>Background</h4>Fluorescence-based biological imaging has been revolutionized by the recent introduction of superresolution microscopy methods. 3D superresolution microscopy, however, remains a challenge as its implementation by existing superresolution methods is non-trivial.<h4>Methods</h4>Here we demonstrate a facile and straightforward 3D superresolution imaging and sectioning of the cytoskeletal network of a fixed cell using superresolution optical fluctuation imaging (SOFI) performed on ...[more]

PMID: 24163789

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