Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.
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ABSTRACT:
SUBMITTER: Coupland P
PROVIDER: S-EPMC3808810 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
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Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.
Coupland Paul P Chandra Tamir T Quail Mike M Reik Wolf W Swerdlow Harold H
BioTechniques 20121201 6
We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ...[more]
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