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A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.

ABSTRACT: Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.

SUBMITTER: Ma M

PROVIDER: S-EPMC3809372 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.

Ma Ming M Ye Adam Y AY Zheng Weiguo W Kong Lei L

BioMed research international 20131003

Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to ...[more]

PMID: 24199189

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