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A novel fexA variant from a canine Staphylococcus pseudintermedius isolate that does not confer florfenicol resistance.

ABSTRACT: Transposon Tn558 integrated in the chromosomal radC gene was detected for the first time in Staphylococus pseudintermedius. It carried a novel fexA variant (fexAv) that confers only chloramphenicol resistance. The exporter FexAv exhibited two amino acid substitutions, Gly33Ala and Ala37Val, both of which seem to be important for substrate recognition. Site-directed mutagenesis that reverted the mutated base pairs to those present in the original fexA gene restored the chloramphenicol-plus-florfenicol resistance phenotype.

SUBMITTER: Gomez-Sanz E

PROVIDER: S-EPMC3811270 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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A novel fexA variant from a canine Staphylococcus pseudintermedius isolate that does not confer florfenicol resistance.

Gómez-Sanz Elena E Kadlec Kristina K Feßler Andrea T AT Zarazaga Myriam M Torres Carmen C Schwarz Stefan S

Antimicrobial agents and chemotherapy 20130826 11

Transposon Tn558 integrated in the chromosomal radC gene was detected for the first time in Staphylococus pseudintermedius. It carried a novel fexA variant (fexAv) that confers only chloramphenicol resistance. The exporter FexAv exhibited two amino acid substitutions, Gly33Ala and Ala37Val, both of which seem to be important for substrate recognition. Site-directed mutagenesis that reverted the mutated base pairs to those present in the original fexA gene restored the chloramphenicol-plus-florfe ...[more]

PMID: 23979755

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