Field-based flow cytometry for ex vivo characterization of Plasmodium vivax and P. falciparum antimalarial sensitivity.
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ABSTRACT:
SUBMITTER: Russell B
PROVIDER: S-EPMC3811473 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
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Field-based flow cytometry for ex vivo characterization of Plasmodium vivax and P. falciparum antimalarial sensitivity.
Russell B B Malleret B B Suwanarusk R R Anthony C C Kanlaya S S Lau Y L YL Woodrow C J CJ Nosten F F Renia L L
Antimicrobial agents and chemotherapy 20130722 10
Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated ...[more]
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