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Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the ?-tubulin complex and directly interacts with GCP3(Alp6).

ABSTRACT: In humans, MOZART1 plays an essential role in mitotic spindle formation as a component of the ?-tubulin ring complex. We report that the fission yeast homologue of MOZART1, Mzt1/Tam4, is located at microtubule-organizing centers (MTOCs) and coimmunoprecipitates with ?-tubulin Gtb1 from cell extracts. We show that mzt1/tam4 is an essential gene in fission yeast, encoding a 64-amino acid peptide, depletion of which leads to aberrant microtubule structure, including malformed mitotic spindles and impaired interphase microtubule array. Mzt1/Tam4 depletion also causes cytokinesis defects, suggesting a role of the ?-tubulin complex in the regulation of cytokinesis. Yeast two-hybrid analysis shows that Mzt1/Tam4 forms a complex with Alp6, a fission yeast homologue of ?-tubulin complex protein 3 (GCP3). Biophysical methods demonstrate that there is a direct interaction between recombinant Mzt1/Tam4 and the N-terminal region of GCP3(Alp6). Together our results suggest that Mzt1/Tam4 contributes to the MTOC function through regulation of GCP3(Alp6).

SUBMITTER: Dhani DK 

PROVIDER: S-EPMC3814152 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6).

Dhani Deepsharan K DK   Goult Benjamin T BT   George Gifty M GM   Rogerson Daniel T DT   Bitton Danny A DA   Miller Crispin J CJ   Schwabe John W R JW   Tanaka Kayoko K  

Molecular biology of the cell 20130904 21

In humans, MOZART1 plays an essential role in mitotic spindle formation as a component of the γ-tubulin ring complex. We report that the fission yeast homologue of MOZART1, Mzt1/Tam4, is located at microtubule-organizing centers (MTOCs) and coimmunoprecipitates with γ-tubulin Gtb1 from cell extracts. We show that mzt1/tam4 is an essential gene in fission yeast, encoding a 64-amino acid peptide, depletion of which leads to aberrant microtubule structure, including malformed mitotic spindles and i  ...[more]

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