Dataset Information

Local connection patterns of parvalbumin-positive inhibitory interneurons in rat primary auditory cortex.

ABSTRACT: In the auditory cortex (AC), GABAergic neurons constitute approximately 15-25% of all neurons. GABAergic cells are present in all sensory modalities and essential for modulating sensory receptive fields. Parvalbumin (PV) positive cells represent the largest sub-group of the GABAergic population in auditory neocortex. We investigated the projection pattern of PV cells in rat primary auditory cortex (AI) with a retrograde tracer (wheat germ apo-HRP conjugated to gold [WAHG]) and immunocytochemistry for PV. All AC layers except layer I contained cells double-labeled for PV and WAHG. All co-localized PV+ cells were within 2 mm of the injection site, regardless of laminar origin. Most (ca. 90%) of the co-localized PV cells were within 500 ?m of the injection site in both dorsal-ventral and rostral-caudal dimension of the auditory core region. WAHG-only cells declined less rapidly with distance and were found up to 6 mm from the deposit sites. WAHG-only labeled cells in the medial geniculate body were in ventral division loci compatible with an injection in AI. Differences in the range and direction of the distribution pattern of co-localized PV+ cells and WAHG-only cells in AI express distinct functional convergence patterns for the two cell populations.

SUBMITTER: Yuan K

PROVIDER: S-EPMC3816087 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Local connection patterns of parvalbumin-positive inhibitory interneurons in rat primary auditory cortex.

Yuan Kexin K Fink Kathren L KL Winer Jeffery A JA Schreiner Christoph E CE

Hearing research 20100625 1-2

In the auditory cortex (AC), GABAergic neurons constitute approximately 15-25% of all neurons. GABAergic cells are present in all sensory modalities and essential for modulating sensory receptive fields. Parvalbumin (PV) positive cells represent the largest sub-group of the GABAergic population in auditory neocortex. We investigated the projection pattern of PV cells in rat primary auditory cortex (AI) with a retrograde tracer (wheat germ apo-HRP conjugated to gold [WAHG]) and immunocytochemistr ...[more]

PMID: 20600741

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Project description:BackgroundInhibitory innervation by parvalbumin (PV) expressing interneurons has been implicated in the onset of the sensitive period of visual plasticity. Immunohistochemical analysis of the development and plasticity of these inhibitory inputs is difficult because PV expression is low in young animals and strongly influenced by neuronal activity. Moreover, the synaptic boutons that PV neurons form onto each other cannot be distinguished from the innervated cell bodies by immunostaining for this protein because it is present throughout the cells. These problems call for the availability of a synaptic, activity-independent marker for PV+ inhibitory boutons that is expressed before sensitive period onset. We investigated whether synaptotagmin-2 (Syt2) fulfills these properties in the visual cortex. Syt2 is a synaptic vesicle protein involved in fast Ca(2+) dependent neurotransmitter release. Its mRNA expression follows a pattern similar to that of PV throughout the brain and is present in 30-40% of hippocampal PV expressing basket cells. Up to now, no quantitative analyses of Syt2 expression in the visual cortex have been carried out.Methodology/principal findingsWe used immunohistochemistry to analyze colocalization of Syt2 with multiple interneuron markers including vesicular GABA transporter VGAT, calbindin, calretinin, somatostatin and PV in the primary visual cortex of mice during development and after dark-rearing.Conclusions/significanceWe show that in the adult visual cortex Syt2 is only found in inhibitory, VGAT positive boutons. Practically all Syt2 positive boutons also contain PV and vice versa. During development, Syt2 expression can be detected in synaptic boutons prior to PV and in contrast to PV expression, Syt2 is not down-regulated by dark-rearing. These properties of Syt2 make it an excellent marker for analyzing the development and plasticity of perisomatic inhibitory innervations onto both excitatory and inhibitory neurons in the visual cortex.

Dataset Information

Local connection patterns of parvalbumin-positive inhibitory interneurons in rat primary auditory cortex.

Publications

Local connection patterns of parvalbumin-positive inhibitory interneurons in rat primary auditory cortex.

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