Ontology highlight
ABSTRACT:
SUBMITTER: Thomas-Chollier M
PROVIDER: S-EPMC3816441 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Thomas-Chollier Morgane M Watson Lisa C LC Cooper Samantha B SB Pufall Miles A MA Liu Jennifer S JS Borzym Katja K Vingron Martin M Yamamoto Keith R KR Meijsing Sebastiaan H SH
Proceedings of the National Academy of Sciences of the United States of America 20131014 44
In addition to guiding proteins to defined genomic loci, DNA can act as an allosteric ligand that influences protein structure and activity. Here we compared genome-wide binding, transcriptional regulation, and, using NMR, the conformation of two glucocorticoid receptor (GR) isoforms that differ by a single amino acid insertion in the lever arm, a domain that adopts DNA sequence-specific conformations. We show that these isoforms differentially regulate gene expression levels through two mechani ...[more]