A single-stranded junction modulates nanosecond motional ordering of the substrate recognition duplex of a group I ribozyme.
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ABSTRACT:
SUBMITTER: Nguyen P
PROVIDER: S-EPMC3817310 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
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A single-stranded junction modulates nanosecond motional ordering of the substrate recognition duplex of a group I ribozyme.
Nguyen Phuong P Shi Xuesong X Sigurdsson Snorri Th ST Herschlag Daniel D Qin Peter Z PZ
Chembiochem : a European journal of chemical biology 20130730 14
Rigid spinning: Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes. ...[more]
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