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Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5.

ABSTRACT: N(6)-methyladenosine (m6A) is a ubiquitous modification found in mammalian mRNA and long noncoding RNA. ALKBH5 is a member of the iron(II)- and 2-oxoglutarate-dependent AlkB oxygenase family and has been shown to catalyze the oxidative demethylation of N(6)-methyladenosine in RNA. The ALKBH5 protein was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.4?Å resolution using synchrotron radiation. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 57.456, b = 83.406, c = 92.909?Å, ? = ? = ? = 90.00° and one molecule in the asymmetric unit.

SUBMITTER: Zhou B

PROVIDER: S-EPMC3818040 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5.

Zhou Bin B Han Zhifu Z

Acta crystallographica. Section F, Structural biology and crystallization communications 20131017 Pt 11

N(6)-methyladenosine (m6A) is a ubiquitous modification found in mammalian mRNA and long noncoding RNA. ALKBH5 is a member of the iron(II)- and 2-oxoglutarate-dependent AlkB oxygenase family and has been shown to catalyze the oxidative demethylation of N(6)-methyladenosine in RNA. The ALKBH5 protein was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.4 Å resolution using synchrotron radiation. The crystals belonged to space group P2(1)2(1)2( ...[more]

PMID: 24192356

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