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Methanol Extract of Artemisia apiacea Hance Attenuates the Expression of Inflammatory Mediators via NF- ? B Inactivation.


ABSTRACT: Artemisia apiacea Hance is one of the most widely used herbs for the treatment of malaria, jaundice, and dyspeptic complaint in oriental medicine. This study investigated the effects of methanol extracts of A. apiacea Hance (MEAH) on the induction of inducible nitric oxide synthase (iNOS) and proinflammatory mediators by lipopolysaccharide (LPS) in Raw264.7 macrophage cells and also evaluated the in vivo effect of MEAH on carrageenan-induced paw edema in rats. MEAH treatment in Raw264.7 cells significantly decreased LPS-inducible nitric oxide production and the expression of iNOS in a concentration-dependent manner, while MEAH (up to 100? ? g/mL) had no cytotoxic activity. Results from immunoblot analyses and ELISA revealed that MEAH significantly inhibited the expression of cyclooxygenase-2, tumor necrosis factor-?, interleukin-1?, and interleukin-6 in LPS-activated cells. As a plausible molecular mechanism, increased degradation and phosphorylation of inhibitory-?B? and nuclear factor-?B accumulation in the nucleus by LPS were partly blocked by MEAH treatment. Finally, MEAH treatment decreased the carrageenan-induced formation of paw edema and infiltration of inflammatory cells in rats. These results demonstrate that MEAH has an anti-inflammatory therapeutic potential that may result from the inhibition of nuclear factor-?B activation, subsequently decreasing the expression of proinflammatory mediators.

SUBMITTER: Ryu JC 

PROVIDER: S-EPMC3819789 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Methanol Extract of Artemisia apiacea Hance Attenuates the Expression of Inflammatory Mediators via NF- κ B Inactivation.

Ryu Ji Choul JC   Park Sang Mi SM   Hwangbo Min M   Byun Sung Hui SH   Ku Sae Kwang SK   Kim Young Woo YW   Kim Sang Chan SC   Jee Seon Young SY   Cho Il Je IJ  

Evidence-based complementary and alternative medicine : eCAM 20131022


Artemisia apiacea Hance is one of the most widely used herbs for the treatment of malaria, jaundice, and dyspeptic complaint in oriental medicine. This study investigated the effects of methanol extracts of A. apiacea Hance (MEAH) on the induction of inducible nitric oxide synthase (iNOS) and proinflammatory mediators by lipopolysaccharide (LPS) in Raw264.7 macrophage cells and also evaluated the in vivo effect of MEAH on carrageenan-induced paw edema in rats. MEAH treatment in Raw264.7 cells si  ...[more]

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