Collection of islets of Langerhans using an equilibrium method.
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ABSTRACT:
SUBMITTER: Koh DS
PROVIDER: S-EPMC3821530 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Publications
Collection of islets of Langerhans using an equilibrium method.
Koh Duk-Su DS Moody Mark M Jo Junghyo J
BioTechniques 20130701 1
Here we present a convenient method for easy hand selection of enzymatically isolated small tissues such as islets of Langerhans. Islets are continuously collected in a micropipette tip connected to a peristaltic pump. After entering the conical micropipette tip, the islets are quickly dragged up by solution flow, but this movement subsequently decreases as the flow rate decreases. Thus, the islets are trapped at a specific height where downward gravitation balances upward buoyancy and the drag ...[more]
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