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Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.

ABSTRACT: BACKGROUND: The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools. OBJECTIVE: We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids. METHODS: Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry. RESULTS: Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts. Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts. Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF- ? , and PCNA expressions in keloids compared to normal tissue. CONCLUSION: Hsp70 is overexpressed in keloid fibroblasts and tissue. The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids.

SUBMITTER: Lee JH

PROVIDER: S-EPMC3821890 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.

Lee Ju Hee JH Shin Jung U JU Jung Inhee I Lee Hemin H Rah Dong Kyun DK Jung Jin Young JY Lee Won Jai WJ

BioMed research international 20131024

<h4>Background</h4>The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools.<h4>Objective</h4>We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids.<h4>Methods</h4>Proteomics ana ...[more]

PMID: 24260741

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Project description:Despite their diminutive size, islets of Langerhans play a large role in maintaining systemic energy balance in the body. New technologies have enabled us to go from studying the whole pancreas to isolated whole islets, to partial islet sections, and now to islet substructures isolated from within the islet. Using a microfluidic nanodroplet-based proteomics platform coupled with laser capture microdissection and field asymmetric waveform ion mobility spectrometry, we present an in-depth investigation of protein profiles specific to features within the islet. These features include the islet-acinar interface vascular tissue, inner islet vasculature, isolated endocrine cells, whole islet with vasculature, and acinar tissue from around the islet. Compared to interface vasculature, unique protein signatures observed in the inner vasculature indicate increased innervation and intra-islet neuron-like crosstalk. We also demonstrate the utility of these data for identifying localized structure-specific drug–target interactions using existing protein/drug binding databases. Graphical Abstract Highlights • We differentiated intra-islet substructures by their proteome profiles in situ.• We demonstrated significant functional differences between vasculature types.• Novel differences in innervation and colocalization of nerves were observed.• We demonstrated a depth of coverage useful for predictive binding discovery assays.• We identified pathways with these techniques that could aid drug characterizations. In Brief Improved sample handling techniques have enabled us to go from studying the whole pancreas, to isolated whole islets, to islet sections, and now to islet substructures isolated from within islet sections. Using a microfluidic nanodroplet-based proteomics platform coupled with laser capture microdissection and field asymmetric waveform ion mobility spectrometry, we present an in-depth investigation of protein profiles from intra-islet features. The protein coverage and expression profiles obtained are sufficient to fully distinguish each of these features while presenting their own unique proteome signatures.

Dataset Information

Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.

Publications

Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.

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