Cooperation of two bifunctional enzymes in the biosynthesis and attachment of deoxysugars of the antitumor antibiotic mithramycin.
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ABSTRACT:
SUBMITTER: Wang G
PROVIDER: S-EPMC3821964 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
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Cooperation of two bifunctional enzymes in the biosynthesis and attachment of deoxysugars of the antitumor antibiotic mithramycin.
Wang Guojun G Pahari Pallab P Kharel Madan K MK Chen Jing J Zhu Haining H Van Lanen Steven G SG Rohr Jürgen J
Angewandte Chemie (International ed. in English) 20120920 42
Two bifunctional enzymes cooperate in the assembly and the positioning of two sugars, D-olivose and D-mycarose, of the anticancer antibiotic mithramycin. MtmC finishes the biosynthesis of both sugar building blocks depending on which MtmGIV activity is supported. MtmGIV transfers these two sugars onto two structurally distinct acceptor substrates. The dual function of these enzymes explains two essential but previously unidentified activities. ...[more]
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