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Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence microscopy.

ABSTRACT: Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein-DNA complexes.

SUBMITTER: Sanchez H

PROVIDER: S-EPMC3822906 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence microscopy.

Sanchez Humberto H Kanaar Roland R Wyman Claire C

Ultramicroscopy 20100324 7

Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility ...[more]

PMID: 20382478

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