Factors affecting relative fitness measurements in pairwise competition assays of human immunodeficiency viruses.
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ABSTRACT:
SUBMITTER: Lanxon-Cookson EC
PROVIDER: S-EPMC3825816 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Factors affecting relative fitness measurements in pairwise competition assays of human immunodeficiency viruses.
Lanxon-Cookson Erinn C EC Swain J Victor JV Manocheewa Siriphan S Smith Robert A RA Maust Brandon B Kim Moon M Westfall Dylan D Rolland Morgane M Mullins James I JI
Journal of virological methods 20130808 1-2
Cell culture growth competition assays of human immunodeficiency virus type 1 (HIV-1) are used to estimate viral fitness and quantify the impact of mutations conferring drug resistance and immunological escape. A comprehensive study of growth competition assays was conducted and identified experimental parameters that can impact measurements of relative fitness including multiplicity of infection, viral input ratio, number, timing and interval of time points used to evaluate selective outgrowth, ...[more]
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