Dataset Information

Roles of JnRAP2.6-like from the transition zone of black walnut in hormone signaling.

ABSTRACT: An EST sequence, designated JnRAP2-like, was isolated from tissue at the heartwood/sapwood transition zone (TZ) in black walnut (Juglans nigra L). The deduced amino acid sequence of JnRAP2-like protein consists of a single AP2-containing domain with significant similarity to conserved AP2/ERF DNA-binding domains in other species. Based on multiple sequence alignment, JnRAP2-like appears to be an ortholog of RAP2.6L (At5g13330), which encodes an ethylene response element binding protein in Arabidopsis thaliana. Real-time PCR revealed that the JnRAP2-like was expressed most abundantly in TZ of trees harvested in fall when compared with other xylem tissues harvested in the fall or summer. Independent transgenic lines over-expressing JnRAP2-like in Arabidopsis developed dramatic ethylene-related phenotypes when treated with 50 µM methyl jasmonate (MeJA). Taken together, these results indicated that JnRAP2-like may participate in the integration of ethylene and jasmonate signals in the xylem and other tissues. Given the role of ethylene in heartwood formation, it is possible JnRAP2-like expression in the transition zone is part of the signal transduction pathway leading to heartwood formation in black walnut.

SUBMITTER: Huang Z

PROVIDER: S-EPMC3827044 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Roles of JnRAP2.6-like from the transition zone of black walnut in hormone signaling.

Huang Zhonglian Z Zhao Peng P Medina Jose J Meilan Richard R Woeste Keith K

PloS one 20131112 11

An EST sequence, designated JnRAP2-like, was isolated from tissue at the heartwood/sapwood transition zone (TZ) in black walnut (Juglans nigra L). The deduced amino acid sequence of JnRAP2-like protein consists of a single AP2-containing domain with significant similarity to conserved AP2/ERF DNA-binding domains in other species. Based on multiple sequence alignment, JnRAP2-like appears to be an ortholog of RAP2.6L (At5g13330), which encodes an ethylene response element binding protein in Arabid ...[more]

PMID: 24265672

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Dataset Information

Roles of JnRAP2.6-like from the transition zone of black walnut in hormone signaling.

Publications

Roles of JnRAP2.6-like from the transition zone of black walnut in hormone signaling.

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