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Monitoring of dynamic microbiological processes using real-time flow cytometry.

ABSTRACT: We describe a straightforward approach to continuously monitor a variety of highly dynamic microbiological processes in millisecond resolution with flow cytometry, using standard bench-top instrumentation. Four main experimental examples are provided, namely: (1) green fluorescent protein expression by antibiotic-stressed Escherichia coli, (2) fluorescent labeling of heat-induced membrane damage in an autochthonous freshwater bacterial community, (3) the initial growth response of late stationary E. coli cells inoculated into fresh growth media, and (4) oxidative disinfection of a mixed culture of auto-fluorescent microorganisms. These examples demonstrate the broad applicability of the method to diverse biological experiments, showing that it allows the collection of detailed, time-resolved information on complex processes.

SUBMITTER: Arnoldini M

PROVIDER: S-EPMC3828236 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Monitoring of dynamic microbiological processes using real-time flow cytometry.

Arnoldini Markus M Heck Tobias T Blanco-Fernández Alfonso A Hammes Frederik F

PloS one 20131114 11

We describe a straightforward approach to continuously monitor a variety of highly dynamic microbiological processes in millisecond resolution with flow cytometry, using standard bench-top instrumentation. Four main experimental examples are provided, namely: (1) green fluorescent protein expression by antibiotic-stressed Escherichia coli, (2) fluorescent labeling of heat-induced membrane damage in an autochthonous freshwater bacterial community, (3) the initial growth response of late stationar ...[more]

PMID: 24244624

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